Double-Stranded RNA Injection Produces Nonspecific Defects in Zebrafish

Nonspecific nucleoside triphosphatase P4 of double-stranded RNA bacteriophage phi6 is required for single-stranded RNA packaging and transcription.

Bacteriophage phi6 has a segmented double-stranded RNA genome. The genomic single-stranded RNA (ssRNA) precursors are packaged into a preformed protein capsid, the polymerase complex, composed of viral proteins P1, P2, P4, and P7. Packaging of the genomic precursors is an energy-dependent process requiring nucleoside triphosphates. Protein P4, a nonspecific nucleoside triphosphatase, has previo...

double-stranded rna viral infection in tehran trichomonas vaginalis isolates.

background : trichomonas vaginalis is a pathogenic protozoon and may be contaminated with dsrna virus called trichomonas vaginalis virus (tvv). the viral infection is an important factor for its patho-genesis and sensitivity to metronidazole. the presence of tvv is associated with qualitative and quantitative expression of cysteine proteinases and surface immunogenic; p270. the purpose of this ...

Double-stranded RNA poses puzzle.

Microglia Recognize Double-Stranded RNA

Expression of yeast L-A double-stranded RNA virus proteins produces derepressed replication: a ski- phenocopy.

The plus strand of the L-A double-stranded RNA virus of Saccharomyces cerevisiae has two large open reading frames, ORF1, which encodes the major coat protein, and ORF2, which encodes a single-stranded RNA-binding protein having a sequence diagnostic of viral RNA-dependent RNA polymerases. ORF2 is expressed only as a Gag-Pol-type fusion protein with ORF1. We have constructed a plasmid which exp...